Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short term studies of protein function. We specifically use this protocol with Lenti-X 293T cells, a cell line optimized for production of lentiviral vectors. Plaquenil and sarcoidosis Plaquenil nursing interventions What is toxic dose of plaquenil Plaquenil eye toxicity Chloroquine is an antimalarial drug that has been used in humans for many years. In recent years, Chloroquine has been shown to inhibit autophagy and induce apoptosis in malignant cells and thus has been tested in various experimental model systems and in human clinical trials. Alexa Fluor R products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer whether the buyer is an academic or for-profit entity. Post-infection chloroquine treatment reduces SARS-CoV infection and spread. Vero E6 cells were seeded and infected as described for Fig. 1 except that chloroquine was added only after virus adsorption. Cells were maintained in Opti-MEM Invitrogen containing. Last Upload: June 10, 2016 Day 0: Seed Lenti-X 293T cells (this cell line is optimized for production of lentiviral vectors) Day 1 (pm): Transfect Cells Day 2 (am): 18h post transfection - Remove media, replace with fresh media Day 3 or more (am): Observe fluorescence, harvest cells, or perform your experiment *Pro-Tips* Different brands and lots of FBS can promote or inhibit transfection. This approach can be adapted for different cell lines and different transfection reagents. Chloroquine invitrogen Premo™ Autophagy Sensor LC3B-GFP BacMam 2.0, Chloroquine Antibody 25G10 Alexa Fluor® 700 NBP1. Chloroquine for hpvChloroquine transfection what isPlaquenil polymyalgia rheumaticaHydroxychloroquine 200mg tablets goodrx Chloroquine enters the red blood cell by simple diffusion, inhibiting the parasite cell and digestive vacuole. Chloroquine then becomes protonated to CQ2+, as the digestive vacuole is known to be acidic pH 4.7; chloroquine then cannot leave by diffusion. Chloroquine - Wikipedia. Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit. C3556 Catalase from human erythrocytes ≥90% SDS-PAGE, buffered aqueous solution, ≥30,000 units/mg protein Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. Chloroquine is the generic form of the brand-name prescription medicine Aralen, which is used to prevent and treat malaria — a mosquito-borne disease caused by a parasite — and to treat. A549 cells treated to induce autophagy and imaged. Left A549 cells were treated with chloroquine and stained with Invitrogen Hoechst 33342, Invitrogen HCS CellMask Deep Red, and an anti-LC3B with Invitrogen Alexa Fluor 488 goat anti-rabbit antibody.